The SAS Super Pinocchio II is a microbial impaction sampler used to test the microbial
quality of compressed air and nitrogen gas in cleanroom environments.
๐๐ฟ๐ผ๐ฐ๐ฒ๐ฑ๐๐ฟ๐ฒ ๐ณ๐ผ๐ฟ ๐จ๐๐ถ๐ป๐ด ๐ฆ๐๐ฆ ๐ฆ๐๐ฝ๐ฒ๐ฟ ๐ฃ๐ถ๐ป๐ผ๐ฐ๐ฐ๐ต๐ถ๐ผ ๐๐
Preparation
๐๐ญ๐๐ซ๐ข๐ฅ๐ข๐ณ๐ ๐๐ช๐ฎ๐ข๐ฉ๐ฆ๐๐ง๐ญ
Autoclave the funnel head and any other parts that will come into direct contact with the
samples to ensure sterility.
๐๐๐ญ๐ฎ๐ฉ
Assemble the unit by attaching the funnel head and connecting the pressure gauge, airflow
meter, and tap as per the manufacturer’s instructions.
Ensure that the contact plate or 90 mm petri dish is securely placed in the plate housing within the funnel head.
๐๐๐ฅ๐๐๐ญ๐ข๐จ๐ง ๐จ๐ ๐๐๐ฆ๐ฉ๐ฅ๐ข๐ง๐ ๐๐ข๐ญ๐
Identify the location in the cleanroom where the compressed air or nitrogen gas sample will
be collected.
Ensure the area is clean, and minimize any disturbance that could lead to contamination.
๐๐จ๐ง๐ง๐๐๐ญ ๐ญ๐ก๐ ๐๐จ๐ฆ๐ฉ๐ซ๐๐ฌ๐ฌ๐๐ ๐๐ข๐ซ ๐๐จ๐ฎ๐ซ๐๐
Attach the compressed air or gas line to the input connection of the SAS Super Pinocchio II
unit.
๐๐๐ ๐ฎ๐ฅ๐๐ญ๐ ๐ญ๐ก๐ ๐
๐ฅ๐จ๐ฐ
Use the built-in flow meter to adjust the air or gas flow rate. Set it to the required flow rate,
typically 100 L/min, as specified in your standard operating procedure (SOP).
Sampling process
๐๐๐ฆ๐ฉ๐ฅ๐ข๐ง๐
Open the tap to allow the compressed air or gas to flow through the unit.
The air or gas will pass through the funnel head and impact the agar surface on the contact
plate or petri dish, depositing any microorganisms present in the sample.
๐๐จ๐ง๐ข๐ญ๐จ๐ซ ๐ญ๐ก๐ ๐ฉ๐ซ๐จ๐๐๐ฌ๐ฌ
Continuously observe the pressure gauge and airflow meter to ensure that the flow rate
remains constant throughout the sampling process.
Sampling for 1 minute at 100 L/min will collect 100 liters of air
10 minutes for 1000 litres of air will collect.
๐๐จ๐ฆ๐ฉ๐ฅ๐๐ญ๐ ๐ญ๐ก๐ ๐๐๐ฆ๐ฉ๐ฅ๐ข๐ง๐
After the required sample volume has been collected, close the tap to stop the flow.
Carefully remove the contact plate or petri dish from the plate housing without disturbing the
agar surface
Transport the sample to the microbiology laboratory for incubation.
Incubate the sampled plates under appropriate conditions like first 20-25ยฐC for 72 hours (03
days) and tranfer plates 30-35ยฐC for 48 hours (02 days)
After incubation, count the number of colony-forming units (CFUs) on each plate.
Record all relevant data including sampling conditions, flow rate, pressure, sampling time,
and microbial counts in your logbook or electronic record system.
