Micropipette calibration is typically performed using the gravimetric method, where the volume dispensed is determined by measuring the weight of distilled water.
๐๐ญ๐๐ฉ-๐๐ฒ-๐๐ญ๐๐ฉ ๐๐ซ๐๐ฏ๐ข๐ฆ๐๐ญ๐ซ๐ข๐ ๐๐๐ฅ๐ข๐๐ซ๐๐ญ๐ข๐จ๐ง
- ๐๐๐๐ฅ๐ข๐ฆ๐๐ญ๐ ๐๐ช๐ฎ๐ข๐ฉ๐ฆ๐๐ง๐ญ: Place the micropipette, distilled water, and tips in the calibration room for at least 2 hours to reach a stable temperature (ideally between 20ยฐC and 25ยฐC)
- ๐๐๐ญ๐ฎ๐ฉ ๐ญ๐ก๐ ๐๐๐ฅ๐๐ง๐๐: Place a small, clean beaker containing 3 mm of distilled water onto an analytical balance and zero the scale.
- ๐๐ซ๐-๐ฐ๐๐ญ ๐ญ๐ก๐ ๐๐ข๐ฉ: Aspirate and dispense the target volume 3โ5 times to humidify the air inside the tip and pipette.
- ๐๐๐ข๐ ๐ก ๐ญ๐ก๐ ๐๐จ๐ฅ๐ฎ๐ฆ๐: : Aspirate the liquid, touch the tip to the inner wall of the beaker at a 45ยฐ angle, and dispense the full amount. Record the weight, re-zero the balance, and repeat this at least 10 times at both the minimum and maximum volumes of the pipette.
- ๐๐๐ฅ๐๐ฎ๐ฅ๐๐ญ๐ ๐๐๐ฌ๐ฎ๐ฅ๐ญ๐ฌ: Convert the recorded weight (mg) to volume (ฮผL) by dividing the weight by the density of water (Z-factor) at the recorded temperature.
