𝐖𝐡𝐲 𝐈𝐬 𝟕𝟎% 𝐈𝐬𝐨𝐩𝐫𝐨𝐩𝐲𝐥 𝐀𝐥𝐜𝐨𝐡𝐨𝐥 (𝐈𝐏𝐀) 𝐔𝐬𝐞𝐝 𝐈𝐧𝐬𝐭𝐞𝐚𝐝 𝐨𝐟 𝟏𝟎𝟎% 𝐀𝐥𝐜𝐨𝐡𝐨𝐥 𝐢𝐧 𝐏𝐡𝐚𝐫𝐦𝐚𝐜𝐞𝐮𝐭𝐢𝐜𝐚𝐥 𝐋𝐚𝐛𝐨𝐫𝐚𝐭𝐨𝐫𝐢𝐞𝐬?

Seventy percent Isopropyl Alcohol (IPA) is used instead of 100% alcohol because water acts as an essential catalyst. Water helps slow evaporation for a longer surface contact time and allows deeper penetration into microbial cell walls.
Pure 100% alcohol coagulates outer cell proteins too fast, creating a protective shell that keeps microorganisms alive. A 70% solution of alcohol takes more time in evaporation from the surface, increasing the contact time. Therefore, 70% isopropyl alcohol fulfils both requirements.

𝐅𝐨𝐥𝐥𝐨𝐰𝐢𝐧𝐠 𝐩𝐨𝐢𝐧𝐭𝐬 𝐬𝐡𝐨𝐮𝐥𝐝 𝐛𝐞 𝐜𝐨𝐧𝐬𝐢𝐝𝐞𝐫𝐞𝐝 𝐰𝐡𝐢𝐥𝐞 𝐮𝐬𝐢𝐧𝐠 𝟕𝟎% 𝐈𝐬𝐨𝐩𝐫𝐨𝐩𝐲𝐥 𝐀𝐥𝐜𝐨𝐡𝐨𝐥:

• Isopropyl Alcohol (IPA) should be prepared daily basis (for use inside Laminar Air Flow (LAF) room) and weekly basis for Hand Sanitization and other disinfectant purposes.
• Freshly Prepared Isopropyl Alcohol should be used in the Production area
• For the preparation of 70%, Isopropyl Alcohol Purified/Mili Q water should be used
• While preparation of 70% IPA measuring should be done accurately
• Isopropyl Alcohol should be validated according to the SOP
• The Bulk Container use for 70% IPA distribution should properly label.

𝐇𝐨𝐰 𝟕𝟎 % 𝐀𝐥𝐜𝐨𝐡𝐨𝐥 𝐖𝐨𝐫𝐤𝐬?
We know 70 % IPA contains 70 % Alcohol and 30 % Water. Water is required to denature the proteins of the cell membrane work and acts as a catalyst and it also increases the contact time of Alcohol within the cell wall of the Organism.

When it contacts to the cell wall it dissolves the plasma membrane of the cell wall because the presence of water it increases the contact time inside the cell wall of bacteria and it slowly penetrates and does not allow to the formation of Coagulates protein layer it goes further and dissolves all protein and Kills Micro-Organism

𝐃𝐢𝐟𝐟𝐞𝐫𝐞𝐧𝐜𝐞

In pharmaceutical microbiology, effective disinfection depends not only on the disinfectant itself—but also on using the right concentration in the right way.